Composite

Part:BBa_K2254001:Design

Designed by: Ching Yuet To   Group: iGEM17_Hong_Kong-CUHK   (2017-08-31)


Toehold Trigger Cloning Tool


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 39
    Illegal NheI site found at 62
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 27
    Illegal BsaI.rc site found at 738


Design Notes

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Source

Its PCR from I20260 in the distribution kit

References